Correlation of HIWI and HILI expression with cancer stem cell markers in colorectal cancer


M. Karczewski, W. Witkiewicz, K. Arczyńska, J. Dubis, A. Frydlewicz, M. Litwin, A. Piotrowska, Correlation of HIWI and HILI expression with cancer stem cell markers in colorectal cancer, Anticancer Research 35 (6), (2015) 3317-3324.

Karczewski M., Witkiewicz W., Arczyńska K., Dubis J., Frydlewicz A., Litwin M., Piotrowska A.,


Background/Aim: Cancer stem cells (CSCs) constitute a sub-population of tumor cells that possess stem cell properties, such as self-renewal and the ability of differentiation. The presence of CSCs is associated with metastatic potential, treatment resistance and poor patient prognosis. Recently, aberrant expression of P-element induced wimpy testis proteins-PIWI (HIWI and HILI) has been identified in various types of tumors. The aim of the study was to evaluate the clinical significance of the HIWI and HILI expression and its relationship with cancer stem cells markers in 72 patients with colorectal carcinoma (CRC). Materials and Methods: The expression level of HIWI and HILI and cancer stem cells markers in paired cancerous and non-cancerous tissues was measured by real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. Immunohistochemistry was performed to confirm the observed changes on mRNA level and detect tissue localization of PIWI proteins. Results: Significantly higher mRNA levels of HIWI and decreased HILI mRNA were measured in colorectal cancer tissues compared to corresponding non-cancerous samples. The changes in HIWI mRNA level in cancer tissues were correlated with OCT4 expression. Positive correlations between HILI level and SOX2 were also observed in cancerous tissues. Conclusion: Our results indicate a reciprocal regulation between HIWI, HILI and some CSCs markers in colorectal cancer.





The Faculty of Environmental Engineering and Geodesy

Institute of Environmental Engineering

Wrocław University of Environmental and Life Sciences

pl. Grunwaldzki 24,
50-363 Wrocław

Project assumptions

The overall goal of the project is to develop an innovative multifactor mathematical model enabling monitoring of bath contamination used in the electropolishing process of austenitic stainless steels. This model will allow optimization and reduction of process costs and will have an impact on reducing environmental pollution during electrolytic polishing of austenitic stainless steels.

The final outcome of the project will consist in the development of a method of monitoring the gradual contamination of the electropolishing bath.


The team deals with research in the field of electrochemistry, wastewater treatment, monitoring and optimization of processes in laboratory and industrial conditions. The diverse experience of individual members of the IonsMonit team is its strength.



Project: “A pioneering model for monitoring pollution of electropolishing process baths (IonsMonit)” financed by the National Center for Research and Development as part of the Lider programme.