Selected endothelial hemostatic markers in patients with peripheral arterial disease after endovascular revascularization and restenosis formation


M. Karczewski, W. Witkiewicz, D. Kotschy, M. Kotschy, J. Kwapisz, L. Masłowski, P. Socha, N. Żuk, Selected endothelial hemostatic markers in patients with peripheral arterial disease after endovascular revascularization and restenosis formation, Postepy Higieny i Medycyny Doswiadczalnej 69 (2015) 905-912. doi:10.5604/17322693.1164409
Karczewski M., Witkiewicz W., Kotschy D., Kotschy M., Kwapisz J., Masłowski L., Socha P., Żuk N.,


Introduction: Surgical and endovascular revascularization of ischemic legs in patients with peripheral arterial disease (PAD) can damage the arterial wall (endothelial and smooth muscle cells). Hemostatic factors released during endothelial dysfunction can lead to restenosis.

Aim: 1. Determination of selected endothelial hemostatic factors in PAD patients and a reference group.

2. Prospective observation of new restenosis appearance in PAD patients after endovascular revascularization.

3. Comparison of selected endothelial hemostatic factors between non-restenotic and restenotic PAD patients.

Patients & methods: 150 PAD patients after endovascular revascularization – 90 men and 60 women, aged 44-88 (mean 65.5) years – were examined. During one-year observation after the revascularization procedures in 38 PAD patients restenosis occurred, when blood samples were also collected. The reference group consisted of 53 healthy persons – 44 men and 9 women, aged 20-56 years. Blood was drawn in the morning into 3.2% sodium citrate at a ratio of 9: 1. Tissue factor (TF), tissue factor pathway inhibitor (TFPI), thrombomodulin (TM), von Willebrand factor (vWF) and tissue plasminogen activator (t-PA) were measured in plasma with commercial tests using the enzyme immunoassay.

Results: In the plasma of PAD patients after revascularization, the concentrations of TF and vWF were significantly higher, TM lower, TFPI and t-PA similar compared to the reference group. Six months after revascularization the level of TF had increased and vWF had significantly decreased. The endothelial hemostatic factors before and after restenosis did not significantly differ except TF, which after restenosis was higher.

Increased TF and vWF levels in PAD patients indicate arterial endothelial cell damage, by atherosclerotic and revascularization processes. In PAD patients with restenosis compared to these patients before restenosis the determined endothelial hemostatic factors, except TF level, did not significantly differ. Perhaps TF participates in restenosis formation.





The Faculty of Environmental Engineering and Geodesy

Institute of Environmental Engineering

Wrocław University of Environmental and Life Sciences

pl. Grunwaldzki 24,
50-363 Wrocław

Project assumptions

The overall goal of the project is to develop an innovative multifactor mathematical model enabling monitoring of bath contamination used in the electropolishing process of austenitic stainless steels. This model will allow optimization and reduction of process costs and will have an impact on reducing environmental pollution during electrolytic polishing of austenitic stainless steels.

The final outcome of the project will consist in the development of a method of monitoring the gradual contamination of the electropolishing bath.


The team deals with research in the field of electrochemistry, wastewater treatment, monitoring and optimization of processes in laboratory and industrial conditions. The diverse experience of individual members of the IonsMonit team is its strength.



Project: “A pioneering model for monitoring pollution of electropolishing process baths (IonsMonit)” financed by the National Center for Research and Development as part of the Lider programme.