The role of the tissue factor and its inhibitor in the development of subclinical atherosclerosis in people living with HIV


M. Karczewski, B. Knysz, W. Kwiatkowska, W. Witkiewicz, K. Arczyńska, K. Barska, The role of the tissue factor and its inhibitor in the development of subclinical atherosclerosis in people living with HIV, PLoS ONE 12 (7), (2017) . doi:10.1371/journal.pone.0181533
Karczewski M., Knysz B., Kwiatkowska W., Witkiewicz W., Arczyńska K., Barska K.,



HIV infection is associated with an increased risk of cardiovascular disease in connection with atherosclerosis and thromboembolic complications. The pathogenesis of atherosclerosis is still unclear in this group of patients. Studies on pathogenesis of atherosclerosis in the general population emphasize the role of the extrinsic pathway of blood coagulation, particularly the tissue factor (TF) and tissue factor pathway inhibitor ( TFPI). The effect of persistent activation of the immune system on enhanced expression of TF on the surface of monocytes in subjects infected with HIV is known to be correlated with the level of HIV RNA in blood serum.

Study aim

The aim of this study was to evaluate the concentration of TF and its inhibitor TFPI in blood plasma, the impact of traditional and non-traditional cardiovascular risk factors on their concentration and the impact of both markers of haemostasis on the severity of subclinical atherosclerosis as assessed by the intima-media measurement of the carotid artery in HIV infected patients.

The study included 121 HIV-infected people with known clinical, immunological and virological status. The control group consisted of 42 healthy individuals, selected in terms of age and sex.

Results and conclusions

Higher concentrations of TF occurred in HIV-infected patients with a low current plasma HIV RNA level, nadir CD4+ T-cell count and longer duration of cumulative antiretroviral treatment. In multivariate analysis, it was the length of cumulative NRTI treatment that impacted on the concentration of TF. The determinants of cardiovascular disease (CVD) risk factors and inflammatory markers did not show any effect on the concentrations of TF.

The TFPI level in HIV-infected patients was significantly higher than in the control group and was negatively correlated with the current level of HIV RNA and nadir CD4+ T-cell count, being higher in patients subjected to antiretroviral treatment. It was shown that the higher the cardiovascular risk and the higher the levels of total cholesterol, low-density lipoprotein cholesterol (LDL) and non-high-density lipoprotein cholesterol (non-HDL), the higher the concentrations of TFPI observed. The levels of TF and TFPI were positively correlated with carotid intima media thickness (cIMT); in the multivariate analysis, TF, non-HDL cholesterol and lifetime smoking (pack-years) independently affected the growth of cIMT. A similar effect on cIMT was demonstrated by TFPI.





The Faculty of Environmental Engineering and Geodesy

Institute of Environmental Engineering

Wrocław University of Environmental and Life Sciences

pl. Grunwaldzki 24,
50-363 Wrocław

Project assumptions

The overall goal of the project is to develop an innovative multifactor mathematical model enabling monitoring of bath contamination used in the electropolishing process of austenitic stainless steels. This model will allow optimization and reduction of process costs and will have an impact on reducing environmental pollution during electrolytic polishing of austenitic stainless steels.

The final outcome of the project will consist in the development of a method of monitoring the gradual contamination of the electropolishing bath.


The team deals with research in the field of electrochemistry, wastewater treatment, monitoring and optimization of processes in laboratory and industrial conditions. The diverse experience of individual members of the IonsMonit team is its strength.



Project: “A pioneering model for monitoring pollution of electropolishing process baths (IonsMonit)” financed by the National Center for Research and Development as part of the Lider programme.